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--- It is a SURE thing ! ---
The Glycine max polyubiquitin (Gmubi) Promoter family
The Gmubi promoter family was selected based on similarities in the protein-coding region of the gene. The Gmubi1-9 coding regions encode perfect tetrameric repeats of the ubiquitin monomer. The Gmubi10 promoter lies upstream of a ubiquitin monomer. Gmubi1-7 promoters are intron-containing while Gmubis8-10 do not contain introns.
| The Gmubi Promoter Family | ||
|
Name
|
Glyma#
|
Size
|
|
Gmubi1
|
Glyma10g39780
|
1449
|
|
Gmubi2
|
Glyma13g17830.1
|
1484
|
|
Gmubi3
|
Glyma20g27950.1
|
1438
|
|
Gmubi4
|
Glyma13g24470.1
|
1430
|
|
Gmubi5
|
Glyma13g24500.1
|
1452
|
|
Gmubi6
|
Glyma07g32020.1
|
1409
|
|
Gmubi7
|
Glyma17g04690.1
|
1453
|
|
Gmubi8
|
Glyma10g05830.1
|
892
|
|
Gmubi9
|
Glyma13g20200
|
1314
|
|
Gmubi10
|
Glyma15g13650.1
|
1355
|
Search Soybean Genome database (Phytozome) using Glyma number.
Extensive characterization of a slightly truncated form of
Gmubi3 is described in:
CM Hernandez-Garcia, AP Martinelli, RA Bouchard, JJ Finer (2009) A soybean
(Glycine max) polyubiquitin promoter gives strong constitutive expression in
transgenic soybean. Plant Cell Rep 28:837-849
Sequence information and expression of the Gmubi family of
promoters are presented in:
Hernandez-Garcia CM,
Bouchard RA, Rushton PJ, Jones ML, Chen X, Timko MP, Finer JJ (2010) High level
transgenic expression of soybean (Glycine
max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis
pipeline. BMC Plant Biology 10:237