Soybean promoter activity is measured over the course of 100 h following introduction of soybean promoter constructs into lima bean cotyledonary tissue (Chiera et al, 2007). Promoter regions, recovered as the ~1.5 kb region upstream of the translational start, are first cloned into pFLEV (Finer Lab Expression Vector) containing the gfp gene. Following introduction via particle bombardment, GFP expression is tracked for 100 h using an automated image collection system (Buenrostro-Nava et al, 2006), and quantified to yield GFP expression profiles.

Buenrostro-Nava MT, Ling PP, Finer JJ (2006) Comparative analysis of 35S and Lectin promoters in transgenic soybean tissue using an automated image acquisition system and image analysis. Plant Cell Reports 25:920-926

Chiera JM, Bouchard RA, Dorsey SL, Park EH, Buenrostro-Nava MT, Ling PP, Finer JJ (2007) Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system. Plant Cell Reports 26:1501-1509