Dr. Renukaradhya J. Gourapura
(Aradhya)

Assistant Professor

Education

* Postdoctoral Fellow, 2002-2008, Indiana University School of Medicine, Indianapolis, Indiana
* Ph.D., 2002, Indian Institute of Science, Bangalore, India
* MVSc, 1994, Veterinary College, Univ. Agri. Sci. Bangalore, India
* DVM, 1991, Veterinary College, Univ. Agri. Sci. Bangalore, India

Research

Mucosal immunology is increasingly gaining attention as an area of great potential for the development of vaccines and immunotherapy. We are interested in understanding mucosal immune responses in the respiratory tract of pigs and other species of food animals and poultry, following viral and bacterial infection and mucosal vaccination. Our goal is to understand host-pathogen interactions, viral pathogenesis and immune evasion mechanisms at mucosal sites. Particular emphasis is on understanding mucosal immune responses mediated through dendritic cells, macrophages, CD4+ and CD8+ lymphocytes, B cells, natural killer (NK) cells, and NKT cells in food animals. We are actively involved in development of mucosal vaccines against infectious and zoonotic pathogens of food animals. Our research is making use of bacterial based and synthetic candidate adjuvants to potentiate the efficacy of live and nanoparticle-based inactivated vaccines administered by intranasal route to protect against porcine reproductive and respiratory syndrome (PRRS) virus and influenza virus. Recently, our lab has discovered functional invariant NKT cells in pigs. The NKT cell plays a pivotal role in generation of cross-protective immunity against genetically divergent influenza viruses. Thus, our laboratory is interested in development of effective mucosal vaccines against influenza with the help of potent NKT cell targeted candidate adjuvants in a pig model.

Selected Publications

http://www.ncbi.nlm.nih.gov/pubmed?term=Renukaradhya

Dwivedi V, C. Manickam, B. Binjawadagi, D. Joyappa, and G.J. Renukaradhya (2012). Biodegradable nanoparticle-entrapped vaccine induces cross-protective immune response against a virulent heterologous respiratory viral infection in pigs. PLOS ONE (In press).

Manickam C, V. Dwivedi, J. Miller, T. Papenfuss, and G.J. Renukaradhya (2012) Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs. Viral Immunology (In press).

Manickam C*, Dwivedi V*, Patterson R, T. Papenfuss, and Renukaradhya G.J (2012). Differential regulation of mucosal immune responses at various mucosal tissues in pigs infected with VR2332 strain of PRRSV. Veterinary Microbiology. 2012 Sep 1. pii: S0378-1135(12)00486-5. doi: 10.1016/j.vetmic.2012.08.021. *Equal authors.

Broxmeyer H.E, K. Christopherson, G. Hangoc, S. Cooper, C. Mantel, G.J. Renukaradhya, and R. R. Brutkiewicz (2012). CD1d Expression on and regulation of murine hematopoietic stem and progenitor cells. Blood, 119(24): 5731-41.

Renukaradhya, G.J., V. Dwivedi, C. Manickam, B. Binjawadagi, D. Benfield (2012). Mucosal vaccines to control porcine reproductive and respiratory syndrome: a new perspective. Animal Health Research Reviews, 13(1):21-37.

Dwivedi V, C. Manickam, B. Binjawadagi, R. Patterson, D. Linhares, M.P. Murtaugh, and G.J. Renukaradhya (2012). Evaluation of immune responses to porcine reproductive and respiratory syndrome virus in pigs during early stage of infection. Virology Journal. 9:45. doi:10.1186/1743-422X-9-45.

Binjawadagi B, V. Dwivedi, C. Manickam, J.B. Torrelles, and G.J. Renukaradhya (2011). Intranasal delivery of an adjuvanted modified live porcine reproductive and respiratory syndrome virus vaccine reduces the ROS production. Viral Immunol. 24(6):1-8.

Dwivedi V, C. Manickam, R. Patterson, K. Dodson, and G.J. Renukaradhya (2011). Intranasal delivery of whole cell lysate of Mycobacterium tuberculosis induces protective immune responses to a modified live porcine reproductive and respiratory syndrome virus vaccine in pigs. Vaccine 29(23):4067-76.

Dwivedi V, C. Manickam, R. Patterson, K. Dodson, M. Murtaugh, J.B. Torrelles, L.S. Schlesinger, and G.J. Renukaradhya (2011). Cross-protective immunity to porcine reproductive and respiratory syndrome virus by intranasal delivery of a live virus vaccine with a potent adjuvant. Vaccine 29(23):4058-66.

Khandelwal, A., G. J. Renukaradhya, M. Rajasekhar, G. Lakshmi Sita, and M. S. Shaila (2010). Immune responses to Hemagglutinin-Neuraminidase protein of peste des petits ruminants virus expressed in transgenic peanut plants in sheep. Vet. Immunol. Immunopathol. 140(3-4):291-296.

Renukaradhya, G.J.*, C. Manickam, M. Khatri, A. Rauf, X. Li, M. Tsuji, G. Rajashekara, and Varun Dwivedi (2010). Functional invariant NKT cells in pig lungs regulate airway hyperreactivity: a potential animal model. J. Clin. Immunol. 31(2):228-39.

Khatri, M., V. Dwivedi, S. Krakowka, C. Manickam, A. Ali, L. Wang, Z. Qin, G. J. Renukaradhya* and Chang-Won Lee* (2010). Swine influenza virus induces acute inflammatory immune responses in pig lungs: a potential animal model to understand human pandemic H1N1 influenza virus. J. Virol., 84(21):11210-11218. * Corresponding authors

Renukaradhya, G.J.*, K.P. Alekseev, K. Jung, Y. Fang and L.J. Saif (2010). Porcine reproductive and respiratory syndrome virus induced immunosuppression exacerbate the inflammatory response to porcine respiratory coronavirus in pigs. Viral Immunol., 23(5):457-466.

Yassine, K.M., C.W. Lee, G.J. Renukaradhya, and Y.M. Saif (2010). Interspecies and intraspecies transmission of influenza A viruses: viral, host and environmental factors. Animal Health Research Reviews, 11(1):53-72. Cambridge University Press.

Jung, K., A. Gurnani, G.J. Renukaradhya, and L.J. Saif (2010). Nitric oxide is elicited and inhibits viral replication in pigs infected with porcine respiratory coronavirus but not porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopath., 136:335-339.

Jung, K., G.J. Renukaradhya, K.P. Alekseev, Y. Fang, Y. Tang, and L.J. Saif (2009). Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J. Gen. Virol., 90:2713-2723.

Renukaradhya, G.J., M.A. Khan, D. Shaji, and R.R. Brutkiewicz (2008). The VSV Matrix Protein Inhibits CD1d-mediated Antigen Presentation through Activation of the p38 MAPK Pathway. J.Virol., 82(24):12535-12542.

Renukaradhya, G.J., M.A. Khan, M. Vieira, W. Du, J. Gervay-Haque, and R.R. Brutkiewicz (2008). Type I NKT cells protect (and Type II NKT cells suppress) the host's innate antitumor immune response to a B-cell lymphoma. Blood, 111(12):5637-5645.

Brutkiewicz, R.R., C.A. Willard, K.K. Gillett-Heacock, M.R. Pawlak, J.C. Bailey, M.A. Khan, M. Nagala, W. Du, J. Gervay-Hague and G. J. Renukaradhya (2007). Protein kinase C d is a critical regulator of CD1d-mediated antigen presentation. Euro. J. Immunol. 37(9):2390-2395.

Larkin, J., G.J. Renukaradhya, V. Sriram, W. Du, J. Gervay-Hague, and R.R. Brutkiewicz (2006). CD44 differentially activates mouse NK T cells and conventional T cells. J. Immunol., 177(1):268-279.

Renukaradhya, G.J., V. Sriram, W. Du, J. Gervay-Hague, L.V. Kaer, and R.R. Brutkiewicz (2006). Inhibition of antitumor immunity by invariant natural killer T cells in a T cell lymphoma model in vivo. Int. J. Cancer, 118(12):3045-3053.

Renukaradhya, G.J., T.J.R. Webb, M.A. Khan, Y.L. Lin, W. Du, J. Gervay-Hague, and R.R. Brutkiewicz (2005). Virus-induced inhibition of CD1d1-mediated antigen presentation: reciprocal regulation by p38 and ERK. J. Immunol., 175(7):4301-4308.

Renukaradhya, G.J., V. Sriram, K. Polakova, G. Russ, and R.R. Brutkiewicz (2004). Development of a quantitative cell-based intracellular ELISA for the screening of B cell hybridoma supernatants: a novel rapid assay to detect positive clones. Hybridoma and Hybridomics, 23:373-379.

Renukaradhya, G.J., K.B. Suresh, M. Rajasekhar and M.S. Shaila (2003). Development of recombinant hemagglutinin competitive ELISA kit for rinderpest sero-surveillance. J Clin. Microbiol., 41(3):943-947.

Renukaradhya, G.J., G. Sinnathamby, S. Seth, M. Rajasekhar and M.S. Shaila (2002). Mapping of B-cell epitopic sites and delineation of functional domains on the Hemagglutinin-Neuraminidase Protein of Peste des petits ruminants virus. Virus Res., 90(1-2):171-185.

Renukaradhya, G.J., S. Isloor and M. Rajasekhar (2002). Epidemiology, zoonotic aspects, vaccination and control / eradication of brucellosis in India. Vet. Microbiol., 90:183-195.

Renukaradhya, G.J., S. Mitra-Kaushik, G. Sinnathamby, M. Rajasekhar and M.S. Shaila (2002). Mapping of B-cell Epitopes of Hemagglutinin Protein of Rinderpest Virus. Virology, 298(2): 214-223.