Dr. Renukaradhya J. Gourapura
* Postdoctoral Fellow, 2002-2008, Indiana University School of Medicine, Indianapolis, Indiana
* Ph.D., 2002, Indian Institute of Science, Bangalore, India
* MVSc, 1994, Veterinary College, Univ. Agri. Sci. Bangalore, India
* DVM, 1991, Veterinary College, Univ. Agri. Sci. Bangalore, India
My laboratory research focus is on mucosal immunology of food animals infected with infectious and zoonotic diseases. Unlike systemic delivery, mucosal delivery of potent vaccines is gaining increased attention to increase the breadth of cross-protective immunity against infections which cause severe disease at mucosal sites. We are interested to understand mucosal immune responses in pigs and other species of food animals to viral and bacterial infections, and to vaccines delivered directly to mucosal sites. Currently, we are evaluating potent bacterial-based and synthetic adjuvants, which were proven effective and safe in other species of animals to use in food animals. Our ongoing studies are aimed to potentiate the cross-protective efficacy of modified live virus and biodegradable nanoparticle-entrapped inactivated virus vaccines of porcine reproductive and respiratory syndrome (PRRS) virus and influenza virus, coadministered intranasally with a potent adjuvant to pigs. Other focus of our study is to understand host-pathogen interactions, viral pathogenesis, and immune evasion mechanisms in food animals infected with viral and bacterial pathogens; and developing a pig model for biomedical research.
Binjawadagi B, V. Dwivedi, C. Manickam, J. Torrelles, K. Ouyang, J.B. Torrelles and G.J. Renukaradhya* (2014). An innovative approach to induce cross-protective immunity against porcine reproductive and respiratory syndrome virus in the lungs of pigs through adjuvanted nanotechnology based vaccination. International Journal of Nanomedicine, 9:1519-1535.
Ouyang K, S. Woodiga, V. Dwivedi, C. Marion, A. Singh, B. Binjawadagi, J. Hiremath, C. Manickam, R. Schleappi, M. Khatri, J. Wu, S. King, and G.J. Renukaradhya* (2014).
Pretreatment of epithelial cells with live Streptococcus pneumoniae has no detectable effect on influenza A virus replication in vitro.PLoS One. 9(3):e90066.
Binjawadagi B, V. Dwivedi, C. Manickam, K. Ouyang, Y. Wu, J. Lee, J.B. Torrelles and G.J. Renukaradhya (2013). Adjuvanted nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits better cross-protective response in pigs. International Journal of Nanomedicine, 9: 679-694.
Olsen C, L. Karriker, C. Wang, G.J. Renukaradhya, B. Binjawadagi, A. Kittawornrat, S. Lizano, J. Coetzee, R. Main, A. Meiszberg, Y. Panyasing, J. Zimmerman (2013). Effect of collection material on pig oral fluid testing results. Vet. Journal, 198(1):158-163.
Renukaradhya G.J, M.A. Khan, R.M. Gallo, D. Shaji, J. Liu, R.R. Brutkiewicz (2013). Forming a complex with MHC class I molecules interferes with mouse CD1d functional expression. PLoS One. 8(8):e72867.
Ouyang K*, B. Binjawadagi*, A. Kittawornrat, C. Olsen, J. Hiremath, N. Elkalifa, R. Schleappi, J. Wu, J. Zimmerman, G.J. Renukaradhya(2013).
Development and Validation of an Assay to detect Porcine Reproductive and Respiratory Syndrome Virus specific Neutralizing Antibody Titers in Pig Oral Fluid Samples. Clin. Vac. Immunol., 20(8):1305-1313. *Equal authors
Dwivedi V, C. Manickam, B. Binjawadagi, and G.J. Renukaradhya (2012). PLGA nanoparticle entrapped killed porcine reproductive and respiratory syndrome virus vaccine helps in viral clearance in pigs. Vet. Microbiol., 166(1-2):47-58.
Dwivedi V, C. Manickam, B. Binjawadagi, D. Joyappa, and G.J. Renukaradhya (2012). Biodegradable nanoparticle-entrapped vaccine induces cross-protective immune response against a virulent heterologous respiratory viral infection in pigs. PLoS One, 7(12):e51794.
Manickam C, V. Dwivedi, J. Miller, T. Papenfuss, and G.J. Renukaradhya (2012). Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs. Viral Immunol., 26(1):102-108.
Manickam C*, V Dwivedi*, R Patterson R, T. Papenfuss, and Renukaradhya G.J (2012). Differential regulation of mucosal immune responses at various mucosal tissues in pigs infected with VR2332 strain of PRRSV. Vet. Microbiol., 162(1):68-77. *Equal authors
Broxmeyer H.E, K. Christopherson, G. Hangoc, S. Cooper, C. Mantel, G.J. Renukaradhya, and R. R. Brutkiewicz (2012). CD1d Expression on and regulation of murine hematopoietic stem and progenitor cells. Blood, 119(24): 5731-41.
Renukaradhya, G.J., V. Dwivedi, C. Manickam, B. Binjawadagi, D. Benfield (2012). Mucosal vaccines to control porcine reproductive and respiratory syndrome: a new perspective. Animal Health Research Reviews, 13(1):21-37.
Dwivedi V, C. Manickam, B. Binjawadagi, R. Patterson, D. Linhares, M.P. Murtaugh, and G.J. Renukaradhya (2012). Evaluation of immune responses to porcine reproductive and respiratory syndrome virus in pigs during early stage of infection. Virology Journal. 9:45.
Binjawadagi B, V. Dwivedi, C. Manickam, J.B. Torrelles, and G.J. Renukaradhya (2011). Intranasal delivery of an adjuvanted modified live porcine reproductive and respiratory syndrome virus vaccine reduces the ROS production. Viral Immunol. 24(6):1-8.
Dwivedi V, C. Manickam, R. Patterson, K. Dodson, and G.J. Renukaradhya (2011). Intranasal delivery of whole cell lysate of Mycobacterium tuberculosis induces protective immune responses to a modified live porcine reproductive and respiratory syndrome virus vaccine in pigs. Vaccine 29(23):4067-76.
Dwivedi V, C. Manickam, R. Patterson, K. Dodson, M. Murtaugh, J.B. Torrelles, L.S. Schlesinger, and G.J. Renukaradhya (2011). Cross-protective immunity to porcine reproductive and respiratory syndrome virus by intranasal delivery of a live virus vaccine with a potent adjuvant. Vaccine 29(23):4058-66.
Renukaradhya, G.J., C. Manickam, M. Khatri, A. Rauf, X. Li, M. Tsuji, G. Rajashekara, and Varun Dwivedi (2010). Functional invariant NKT cells in pig lungs regulate airway hyperreactivity: a potential animal model. J. Clin. Immunol. 31(2):228-39.
Khatri, M., V. Dwivedi, S. Krakowka, C. Manickam, A. Ali, L. Wang, Z. Qin, G. J. Renukaradhya* and Chang-Won Lee* (2010). Swine influenza virus induces acute inflammatory immune responses in pig lungs: a potential animal model to understand human pandemic H1N1 influenza virus. J. Virol., 84(21):11210-11218. *Corresponding authors
Renukaradhya, G.J.*, K.P. Alekseev, K. Jung, Y. Fang and L.J. Saif (2010). Porcine reproductive and respiratory syndrome virus induced immunosuppression exacerbate the inflammatory response to porcine respiratory coronavirus in pigs. Viral Immunol., 23(5):457-466.
Jung, K., G.J. Renukaradhya, K.P. Alekseev, Y. Fang, Y. Tang, and L.J. Saif (2009). Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J. Gen. Virol., 90:2713-2723.
Renukaradhya, G.J., M.A. Khan, D. Shaji, and R.R. Brutkiewicz (2008). The VSV Matrix Protein Inhibits CD1d-mediated Antigen Presentation through Activation of the p38 MAPK Pathway. J.Virol., 82(24):12535-12542.
Renukaradhya, G.J., M.A. Khan, M. Vieira, W. Du, J. Gervay-Haque, and R.R. Brutkiewicz (2008). Type I NKT cells protect (and Type II NKT cells suppress) the host's innate antitumor immune response to a B-cell lymphoma. Blood, 111(12):5637-5645.
Brutkiewicz, R.R., C.A. Willard, K.K. Gillett-Heacock, M.R. Pawlak, J.C. Bailey, M.A. Khan, M. Nagala, W. Du, J. Gervay-Hague and G. J. Renukaradhya (2007). Protein kinase C d is a critical regulator of CD1d-mediated antigen presentation. Euro. J. Immunol. 37(9):2390-2395.
Renukaradhya, G.J., V. Sriram, W. Du, J. Gervay-Hague, L.V. Kaer, and R.R. Brutkiewicz (2006). Inhibition of antitumor immunity by invariant natural killer T cells in a T cell lymphoma model in vivo. Int. J. Cancer, 118(12):3045-3053.
Renukaradhya, G.J., T.J.R. Webb, M.A. Khan, Y.L. Lin, W. Du, J. Gervay-Hague, and R.R. Brutkiewicz (2005). Virus-induced inhibition of CD1d1-mediated antigen presentation: reciprocal regulation by p38 and ERK. J. Immunol., 175(7):4301-4308.