Publications

 

 

1972 (MS Thesis)

Discovery (with advisor, Dr. E.H. Bohl) of Gut-Mammary SIgA Immulogic Axis in Pigs as One of First Descriptions of Common Mucosal Immune System

 

E. Bohl and L. J. Saif

Saif, L. J., E. H. Bohl and R. K. P. Gupta.  l972.  Isolation of porcine immunoglobulins and determination of the immunoglobulin classes of transmissible gastroenteritis viral antibodies.  Infect. & Immun. 6:600‑609.

 

Bohl, E. H., R. K. P. Gupta, L. W. McCloskey and L. J. Saif.  l972. Immunology of transmissible gastroenteritis.  JAVMA 160:543‑549.

 

 

1980 - 2004

Discovery of First Non-Group A Rotavirus

Discovery of First Porcine Enteric Calicivirus

Adaptation of Fastidious Enteric Viruses to Cell Culture

  • Group A and C Rotaviruses

  • Growth of only enteric Calicivirus adapted cell culture using a novel approach (intestinal contents or bile acids in culture medium)

 

 
 

 

    

Human Group A Rotavirus   Porcine Group C Rotavirus

 

   Porcine Enteric Calicivirus- Sapovirus   

 

 

  Porcine Astrovirus
1980- First Group A rotavirus grown in cell culture in collaboration with NIH co-workers. R. Wyatt, E. Bohl, K. Theil, & L. Saif  et al.

Wyatt, R. G.; James, W. D.; Bohl, E. H.; Theil, K. W.; Saif, L. J.; Kalica, A. R.; Greenberg, H. B.; Kapikian, A. Z., and Chanock, R. M. Human rotavirus type 2: cultivation in vitro. Science. 1980; 207:189-91.

 

1987- First Group C rotavirus grown in cell culture. L. A. Terrett & L. J. Saif  

Terrett, L. A. and Saif, L. J. Serial propagation of porcine group C rotavirus (pararotavirus) in primary porcine kidney cell cultures. J Clin Microbiol. 1987; 25:1316-9.

 

1988- First enteric calicivirus grown in cell culture. W. T. Flynn & L. J. Saif

Flynn, W. T. and Saif, L. J. Serial propagation of porcine enteric calicivirus-like virus in primary porcine kidney cell cultures. J Clin Microbiol. 1988; 26:206-12.

 

 

  1. Virus Passage in germfree pigs amplified the virus, permitting growth in cell culture.
  2. Virus growth in cell culture is essential to study viruses and important to develop vaccines.
  3. The disease potential of each virus was confirmed by exposure of germfree pigs.

 

2004- In collaboration with NIH colleagues, bile acids identified as a factor in intestinal contents enhancing cell culture growth of an enteric calicivirus through down-regulation of STAT-1. K. O. Chang & L. J. Saif

Chang, K.O., S.V. Sosnovtsev, G. Belliot, Y. Kim, L.J. Saif, and K.Y. Green. 2004. Bile acids are essential for porcine enteric calicivirus replication in association with down-regulation of stat1. Proc. Natl. Acad. Sci USA 101:8733-8.
 

  

 

 

1981

Discovery of Ohio (OH) strain of Bovine Torovirus

Discovered from OARDC Feedlot Cattle

 

 

 

 

Bovine Torovirus

G. N. Woode and L. J. Saif, et al.

Woode, G. N.; Saif, L. J.; Quesada, M.; Winand, N. J.; Pohlenz, J. F., and Gourley, N. K. Comparative studies on three isolates of Breda virus of calves. Am J Vet Res. 1985;46:1003-10.
 

  

 

2002

Discovery of New Genogroup or Genus of Bovine Enteric Calicivirus

Identified from Young Calves- NB-BEC Strain

 

Bovine Enteric Calicivirus

J. Smiley and L. Saif, et al.

Smiley, J. R.; Chang, K. O.; Hayes, J.; Vinje, J., and Saif, L. J. Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus. J Virol. 2002;76:10089-98.

 

Disease potential of each virus verified by oral exposure of germfree calves
 

 

Home