Compare Alignment Ver.1
Compare Alignment compares the result of a ClustalW alignment output file for dissimilarities as shown below:
MSV-K .............A C G C A T A
MSV-N .............A C G G A T T
MSV-Z .............T C G C A T A
The output of Compare Alignment is a file that can be imported into Excel that shows the results after the aligned sequences have been compared.
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ORF Extractor
ORF Extractor extracts Open Reading Frames from contigs of any size. ORFs can be extracted in all six frames. ORFs length, start and stop codons can be selected. All ORFs or selected ORFs are saved in an output file that can be used for Blast searches.
>Contig50_6R Forward 2-34, 33
TATTGTTTGAGTTATTT….
>Contig50_10R Forward 639-1433, 795
ATGCAACAGCGAGGTTATAAAGGA….
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Hit Extractor
Hit Extraction parses the Blast output file and extracts a number of hits, defined by the user, for each Fasta entry.
Extracting 1 hit(s) from each FASTA file.
Petunia-C2H4-1-A01.g
gb|AC04.1|F13M7 Arabidopsis thal 50 4e-004
Petunia-C2H4-1-A02.g
GB|L219.1|PETAC Petunia hybrid 31 5e-083
Petunia-C2H4-1-A03.g
*** No Hits Found ***
The text above is a sample of Hit extraction’s output.
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Sequence Order Search, SOS
SOS identifies sense and anti-sense hits in a Blast output file. An example of an output file is shown below: notice the decreasing indices, (63 – 122) and (183 – 242), of the subject sequence
Query: 63 .cacggcgcccgctgctgtggccgg 122
.................||||||||||||||||||||||||||||||
Sbjct: 161 cacggcgcccgctgctgtggccgg 120
Query: 183 cctatcggacgtgttccgtgcca 242
..................||||||||||||||||||||||||||||
Sbjct: 281 cctatcggacgtgttccgtgcca 240
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PexFinder Ver.2
PexFinder ver.2 searches the SignalP output file with users defined criteria for specific extracellular signal peptide sequences, and extracts the files. Below is a sample SignalP input with prediction and range bet. 10 and 40:
# Most likely cleavage site between pos. 23 and 24: GEA-LS
SignalP-HMM result:
>PI001E12.XT7.SEQ
Prediction: Signal anchor
Signal peptide probability: 0.008
PexFinder2 Fasta output file:
>PI001E12.XT7.SEQ
MEMSSKIACFIVLCMSKIACFI…
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EleNor
EleNor (Electronic Northern) analyses various EST databases for the frequency of expression of specific genes. Some features of this program are still being developed.
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