Lab Intro

We study the ecology of plant-associated microorganisms, especially those that contribute to the development and suppression of plant diseases. The specific goals of the research are to (i) characterize the biogeography, diversity, and activities of genes and microorganisms associated with plant growth promotion and plant disease suppression, (ii) determine the impact of different farm management practices and other environmental variables on the occurrence and activities of those functionally important microbial populations, and (iii) develop cost-effective biological control strategies that improve agricultural sustainability, productivity, and profitability. 

Current research involves molecular characterization of microbial populations that colonize plants and their responses to different farm management practices.  Some of the methods employed include dual culture bioassays, electron microscopy, DNA sequencing, quantitative PCR (qPCR), suppressive subtractive hybridization (SSH), and molecular profiling of ribosomal genes using terminal restriction fragment length polymorphism (T-RFLP) analyses.  Initially, our lab focused on studying the ecology and biocontrol efficacy of DAPG-producing Pseudomonas spp. as well as several Bacillus  spp.  That work continues and is being supplemented by parallel studies on recently discovered species that contribute to general soilborne disease suppression and root diseases in various contexts.  The ultimate goal of this research is to improve the biological control of plant diseases through the development of biopesticides suitable for use in organic as well as conventional agriculture.

            Future avenues of research will characterize the biogeography of other plant-associated microbes in agricultural contexts, the disease-reducing molecular mechanisms expressed by different biocontrol bacteria, and the interactions among host, biocontrol agent, and pathogen.  Given the founding mission of this Land Grant University, my chosen research systems include three of Ohio’s most valuable and widely-planted crops (i.e. corn, soybeans, wheat, and tomatoes) as well as other high-value fruit, vegetable, and nursery crops. And, all are aimed at improving the profitability and sustainability of small- to medium-sized family farms, especially those who choose to operate within a certified organic framework.

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