Objective A

 

 

 

 

NC-213

 

 

 

 

Determine the effects of genetic traits, abiotic environmental conditions, and handling practices on the quality of cereals and oilseeds

 

 



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                                                                                 NC-213 Progress Report for 2002

From:   Iowa State University
            Department of Agricultural and Biosystems Engineering

By:       Hurburgh, Jr., C.R.
            Brumm, T.J.

NC-213 Objective:      A                                                                                                                            Procedure:     1a

Project Objectives:       Maintain a database of grain quality information.

Results for 2002: Since 1986, Iowa State University (ISU) and the American Soybean Association (ASA) have been surveying the quality of new crop soybean harvests. In response to a mailed request producers, representing all 29 soybean production states, provided samples of 2001 crop soybeans for analysis. Samples were analyzed for protein and oil contents with an Infratec near-infrared instrument (Foss North America, Eden Prairie, Minn.). Other sources provided data on yield and physical quality (U.S. Grade factors) of U.S. soybeans. Data was organized by state and region (groups of states). As in previous years, the data was collected to support international marketing efforts.

The United States produced 2.73 billion bushels (73.4 million metric tons) of soybeans according to the November 1 USDA production estimates. Soybean yields, at 37.5 bushels per acre, were below the 25-year trendline of increases. Average U.S. protein and oil contents (Table 1) are both above long-term averages (Table 2) of 35% protein and 19% oil. These soybeans will produce – again on average – 43.2 lbs of 48% protein meal and 11.4 lbs of oil per bushel. The combination of oil and protein, indicative of total value, was the highest in the 17 years of the survey.

The variability (standard deviation) within states, regions, and the U.S. was significantly lower than 2001. More consistent protein and oil contents in export cargoes can be expected relative to last year. The north to south protein pattern (lower north, higher south) was again evident in 2002 but the spread was the lowest in several years.

Plans for 2003:     Continue the survey. Expand the amino acid component. Publish historic data and trends.

Publications:
Hurburgh, C.R., Jr. 2002. Quality of the US 2001 soybean crop. American Oil Chemists Society, Inform 13(5):394.

Brumm, T.J. and C.R. Hurburgh, Jr. 2002. Quality of the 2002 Soybean Crop from the United States. United Soybean Board, St. Louis, MO and Iowa Grain Quality Initiative, Ames, IA. http//www.iowagrain.org. Presented in Tokyo, Seoul, Taipei, and Shanghai. December 4-12, 2002.

Table 1. American Soybean Association 2002 soybean quality survey data.



Table 2. Summary of Yield and Quality Data for U.S. Soybeans.



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                                                                                NC-213 Progress Report for 2002


From:     The Ohio State University
              Ohio Agricultural Research and Development Center
              Department of Horticulture and Crop Science

By:         Pratt, R.C.
              Thomison, P.R.
              Beuerlein, J.E.

NC-213 Objective:     A                                                                                                                      Procedure:     1a

Project Objectives: Determine the effects of genetic traits, climatic factors, agronomic practices, pest populations, machine harvesting, and drying on the quality of cereals and oilseeds.

Results for 2002: One grain sample of each soybean cultivar entered in 1 test in the appropriate maturity region, and 3 samples of each maize hybrid in 1 test within each of 3 regional tests, were analyzed using the Tecator 1225 whole grain near-infrared transmittance analyzer and Compositional Systems System One calibrations. The 2001 corn compositional values reported in the 2001 Annual Report were erroneous. The correct values are reported in Table 1. The 2002 corn (n=358), and soybean (n=192) compositional values from the respective state variety performance tests are reported in Tables 2-3. The data show corn compositional values were higher in 2002 than in 2001 at all locations. Corn compositional values were highest in the Norwest region for protein and starch. Northwest region values were equal to North central - Northeastern regional sites in oil composition, while still higher than Southwestern - West Central regional tests. Soybean oil and protein concentrations were both higher in 2002 than in 2001. Roundup Ready and conventional soybean grain quality characteristics were nearly identical, except that seed size was greater for the normal varieties

Plans for 2003: A database documenting regional and seasonal variations in compositional data in Ohio will be generated.

Publications:
2002 Ohio Corn and Soybean Performance Trials. Ohio’s Country Journal, December 2002. Dept. of Horticulture and Crop Science: Soybean Series 212 and Corn Series 215. (Data will also be put on the web at: www.ag.ohio-state.edu/~perf)

Issues: New market opportunities are being created by the sale of specialty hybrids with value-added traits such as elevated grain protein or oil composition. Objective evaluations are needed so that producers will know the compositional characteristics, as well as the agronomic characteristics, of cultivars. Information is also needed to ascertain trends attributable to season or region of production.

What Was Done: Grain samples of varieties included in soybean and maize performance tests were analyzed and reported. Producers and seedsmen now have an information base from which to evaluate the value of different cultivars for various end-uses.

Impacts: Greater awareness of grain compositional traits of varieties has been created. Linkages between producers and end-users of value-added crops have been fostered.

Contacts:
Rich Pratt Peter R. Thomison
NC-213 Ohio Representative Corn Performance Test
Horticulture and Crop Science Horticulture and Crop Science
OSU-OARDC OSU-OARDC
tel. 330-263-3972 fax. 330-263-3887 tel. 614-292-2373 fax. 614-292-7162
email Thomison.1@osu.edu

Jim Beuerlein
Soybean Performance Test
Horticulture and Crop Science, OSU
tel. 614-292-9080 fax. 614-292-7162




Table 1. Grain Quality of Corn Hybrids in Ohio, 2001.

Region

-------- Number ---------

-- Protein % --

---- Oil % ----

---- Startch % ----

 

hybrids

locations

mean

range

mean

range

mean

range

Southwestern - West Central

122

1

7.5

6.3-8.4

3.3

3.0-4.0

57.2

53.3-59.9

Northwestern

124

1

8.5

7.6-9.5

3.8

3.3-4.2

61.3

59.9-63.1

Northcentarl - Northeastern

96

1

8.7

7.5-10.2

3.8

3.3-4.3

61.2

60.0-62.6

Compositional data are expressed at 15.0% moisture basis.


 



Table 2. Grain Quality of Corn Hybrids in Ohio, 2002.

Region

-------- Number ---------

-- Protein % --

---- Oil % ----

---- Startch % ----

 

hybrids

locations

mean

range

mean

range

mean

range

Southwestern - West Central

128

1

8.5

7.4-9.6

4.1

3.3-4.6

63.5

61.6-66.3

Northwestern

141

1

9.0

7.4-11.2

4.4

3.8-5.0

64.5

61.4-67.1

Northcentarl - Northeastern

89

1

8.3

7.2-9.5

4.0

3.6-4.6

64.6

62.3-67.0

Compositional data are expressed at 15.0% moisture basis.




Table 3. Grain Quality of Soybean Cultivars in Ohio, 2002.

Entry Type

 

--- Protein % ---

--- Oil % ---

--- Seeds/Lb. ---

 

No. of entries

mean

range

mean

range

mean

range

 

 

 

 

 

 

 

 

Roundup Ready

168

41.8

40.1-43.8

21.2

19.6-23.1

2923

 

Normal

24

41.8

39.1-43.3

21.2

20.5-23.1

2664

 

Compositional data are expressed at 13.0 % moisture basis.

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                                                                                     NC-213 Progress Report for 2002



From:    Texas A&M University
             Soil & Crop Sciences Department

By:        Rooney, L.W.*
             Betran, J.
             Waniska, R.D.
             Jones, E.

NC-213 Objective:     A                                                                                                                           Procedure:     1a

Project Objectives:      Evaluate physical, chemical and processing properties of sorghum and develop improved food quality sorghums.

Determine differences in alkaline cooking properties of corn and sorghum that relate to kernel characteristics and physical properties.

Determine alkaline cooking properties of corn hybrids and improved value added types. Improve the aflatoxin and processing quality of corn through breeding. Evaluate factors affecting staling of corn tortillas and how to control it.

Determine milling and baking quality of wheat breeding lines. Define the attributes of wheat flours with excellent quality for flour tortillas.

Results for 2002: New white and red tan plant hybrids with promising properties were identified in sorghum hybrid trials grown across the sorghum belt. Several new earlier maturity food hybrids are nearing commercial reality.

Whole sorghum snacks were produced by low cost friction extrusion. They have excellent properties and can be flavored with mild to strong seasonings. The hybrids used are derived from ATx631XRTx436 released from TAES several years ago. Market development in Asia and Mexico by the US Grains Council with assistance from this project was continued.

NIR and single kernel hardness methods were used to evaluate sorghum quality. Calibration equations for starch, protein and moisture from more than 155 whole kernel sorghum samples were developed for a diode array NIR. The single kernel hardness test was significantly correlated to decortication yields of sorghum.

Milling evaluation of food type sorghums includes adjustment of the milled product yields to a constant acceptable color measured with the L value. This is the most efficient method of selecting for milling properties where color is important. Some bright white sorghums produce acceptable color products at 90% or higher yields.

A blend containing high antioxidant sorghum bran, soluble and insoluble dietary fiber from barley and omega-3 fatty acids from flax seed produced bread with a natural brown crumb color and excellent taste and texture. The sorghum bran has ORAC values equal to or higher than fruits and berries.

Methods to quantify flavonoids and catechins and their antioxidant potentials are being utilized to determine which milling fractions and cultivars should be used as nutraceutical ingredients.

Higher levels and increased retention of antifungal proteins (chitinase and sormatin) in caryopses of sorghum at physiological maturity and combine harvest maturity improve grain mold resistance. Sorghums inoculated with fungal pathogens had decreased seed germination even though changes in grain appearance due to molding were similar and minor. The antifungal protein levels vary depending upon genotype, inoculation, and grain mold resistance.

Wheat samples (nearly 2,000) were evaluated for milling and baking quality in collaboration with the wheat improvement program where approximately one new variety has been released annually for the last 30 years.

Quality protein maize inbreeds released by the corn improvement program have good food processing properties and higher lysine and tryptophan content. Progress has been made to develop corn lines with improved resistance to aflatoxin.

Some combination of protein quality and content characterizes the properties of wheat flours that produce excellent tortillas. Flours with good bread baking properties usually do not produce good tortillas. The hot press operation is critically important for tortilla quality evaluation so test methods should involve sufficient pressure and heat to mimic the commercial situation.

Elite breeding lines and commercial wheat varieties were evaluated for tortilla properties using a large pilot scale press procedure.

The retention of tortilla flexibility during storage (freshness) was improved by addition of wheat protein isolates to the formula; however, these treatments caused undesirable smaller diameter tortillas. However, utilization of selected protein isolates significantly improved the performance of flours that produce tortillas with short shelf stabilities and large diameters. The combination of protein isolate gives these weaker flours the ability to produce tortillas with optimum attributes.

Staling of corn tortillas is reduced by use of specific amylase enzymes in combination with CMC and barley flour and concentrates. Low levels of barley flour give significant improvement in texture of table tortillas. A concentrate of barley flour containing 70% b-glucans gave positive improvement in tortilla texture and did not affect the color or flavor of the tortillas.

Leavening systems for flour tortillas are critically important. Optimum combinations of sodium aluminum phosphate and sodium aluminum sulfate produce tortillas with significantly improved properties.

Plans for 2003:     Evaluate factors affecting processing quality of corn and sorghum. Compare extrusion properties of sorghum with corn and rice. Develop calibration equations for use of NIR on whole grains of sorghum, maize and their products, e.g., masa, chips, tortillas. Determine factors affecting the staling of flour and corn tortillas. Determine quality of wheat samples emphasizing early generation progeny. Evaluate wheat flours and other ingredients for tortilla quality.

Publications:
Bandyopadhyay, R., D.R. Butler, C.R., Little, and R.D. Waniska. 2002. Sorghum grain mold: through the 1990s into the new millennium. Sorghum and Millet Diseases 2000, Leslie, J.F. (ed.), Iowa State University Press. (In press.)

Dombrink-Kurtzman, M.A. and L.W. Rooney. 2002. Effect of nixtamalization on fumonisin contaminated corn for production of tortillas. Chapter 15 in Bioactive Compounds in Foods: Effects of Food Processing, Tung-Ching Lee and Chi-Tang Ho, (eds.) American Chemical Society Symposium Series 816:206-217.

Ribaut, J.M., M. Bänziger, K. Dreher, G.O. Edmeades, H.J. Hoisington and C. Jiang. 2002. Use of molecular markers in plant breeding: drought tolerance improvement in tropical maize in “Quantitative Genetics, Genomics, and Plant Breeding”, Manjit S. Kang (ed.), chapter 7, CABI Publishing, Wallingford, UK. (In press.)

Rodriguez-Ballesteros, O.R., R.A. Frederiksen, A. Mansuetus, G.N. Odvody, R.D. Waniska, and D.T. Rosenow. 2002. Free and bound phenolic acids in mature sorghum caryopses as affected by inoculation with Fuisarium thapsinum, chapter in Sorghum and Millet Diseases 2000, Leslie, J.F. (ed.), Iowa State University Press. (In press.)

Waniska, R.D. and L.W. Rooney. 2002. Sorghum grain quality for increased utilization. Sorghum and Millet Diseases 2000, Leslie, J.F. (ed.), Iowa State University Press. (In press.)

Waniska, R.D., F.P. Bejosano, A. Chandrashekar, J. Jayaraman, J. Jeoung, S, Krishnaveni, G.H. Liang, S. Muthukrishnan, S. and R.T. Venkatesha. 2002. Antifungal proteins and other mechanisms in the control of sorghum stalk rot and grain mold. Sorghum and Millet Diseases 2000, Leslie, J.F. (ed.) Iowa State University Press. (In press.)

Adams, J.L. and R.D. Waniska. 2002. Effects of the Amount and Solubility of Leavening Compounds on Flour Tortilla Characteristics. Cereal Foods World, 47(2):60-64.

Awika, J.M., L.W. Rooney and E.L. Suhendro. 2002. Milling value of sorghums compared by adjusting yields to a constant product color. Cereal Chem.79 :(2):249-251.

Betrán, F.J. and T. Isakeit. 2002. Aflatoxin contamination of early, intermediate, and late maturing maize hybrids. Mycopathologia 155:86.

Betrán, F.J., T. Isakeit, K. Mayfield and G. Odvody. 2002. Maize germplasm evaluation for aflatoxin resistance in Texas. Mycopathologia 155:81.

Betran, .J., T. Isakeit and G. Odvody. 2002. Aflatoxin accumulation of white and yellow inbreeds in diallel crosses. Crop Science 42: 1894-1901.

Bhatnagar, S., F.J. Betrán and D. Transue. 2002. Aflatoxin resistance of subtropical/tropical quality protein maize hybrids in Texas. Mycopathologia 155:85.

Cheewapramong, P., E.W. Lusas, M.N. Riaz and L.W. Rooney. 2002. Use of partially defatted peanut flour in breakfast cereal flakes. Cereal Chem. 79(4):586-592.

Kelekci, N., S. Pascut and R.D. Waniska. 2002. The effects of storage temperatures on the staling of wheat flour tortillas. J. Cereal Sci. (In press.)

Seetharaman, K., N. Chinnapha, R.D. Waniska and P. White. 2002. Changes in textural, pasting and thermal properties of wheat buns and tortillas during storage. J. Cereal Sci. 35:215-223.

Waniska, R.D., J.L. Adams and R.A. Graybosch. 2002. Effect of partial waxy wheat on processing and quality of wheat flour tortillas. Cereal Chemistry 79(2):210-214.

Betrán, F.J., S. Bhatnagar, R. Ganunga, T. Isakeit, D. Makumbi, K. Mayfield, K., D. Pietsch and D. Transue. 2002. Response of white food corn to aflatoxin accumulation. In Proc. of the Aflatoxin/Fumonisin Workshop 2002. October 23-25, 2002. San Antonio, TX.

Pascut, S. December 2002. Effects of added wheat proteins on processing and quality of flour tortillas. MS Thesis. Texas A&M University, College Station, Texas. 93 pp.

Acosta, D., M. Barron, M. Riaz, L. Rooney and R. Waniska. 2002. Comparative analysis of rice and sorghum extrusion properties. 87th Annual Meeting, October 13 17, Montreal, Quebec. http://www.aaccnet.org/meetings/2002/abstracts/a02ma340.asp

Arora, S., S. Pascut and R. Waniska. 2002. Use of tortilla baking test to evaluate wheat cultivars. AACC 87th Annual Meeting, October 13-17, Montreal, Quebec. http://www.aaccnet.org/meetings/2002/abstracts/a02ma387.asp

Awika, J.M., D. Gualberto, and L.W. Rooney. 2002. Properties of white food sorghums grown in different environments. AACC 87th Annual Meeting, October 13 17, Montreal, Quebec. http://www.aaccnet.org/meetings/2002/abstracts/a02ma378.asp

Bueso, F., R. Moreira, L.W. Rooney, L. Silva and R.D. Waniska. 2002. Anti staling properties of a maltogenic amylase on corn tortillas. AACC 87th Annual Meeting, October 13 17, Montreal, Quebec.http://www.aaccnet.org/meetings/2002/abstracts/a02ma385.asp

Bueso, F., R. Moreira, L.W. Rooney and R.D. Waniska. 2002. Anti-staling properties of a maltogenic amylase on corn tortillas. IFT Annual Meeting and Food Expo, June 15-19, Anaheim, CA, http://ift.confex.com/ift/2002/techprogram/paper_13692.htm.

Garza-Casso, J.B., N. Maranphal, L.W. Rooney and R.D. Waniska. 2002. Phosphate salt affects wheat flour tortilla properties. June 15-19, Anaheim, CA.
http://ift.confex.com/ift/2002/techprogram/ paper_14049.htm.

Garza-Casso, J.B., L.W. Rooney and R.D. Waniska. 2002. Fumaric acid induced changes in pH of flour tortillas. 2002 Agricultural Program Conference Graduate Poster Contest. College Station, Texas.

Leal Diaz, A., J. Betran, L. Rooney and R. Waniska. 2002. Alkaline cooking properties of quality protein maize. AACC 87th Annual Meeting, October 13 17, Montreal, Quebec.
http://www.aaccnet.org/meetings/2002/abstracts/a02ma383.asp

McDonough, C.M., J. Awika, L. Dykes, L.W. Rooney and R.D. Waniska. 2002. False positives for tannin sorghum in non tannin sorghum using the bleach test. AACC 87th Annual Meeting, October 13 17, Montreal, Quebec. Quebec.
http://www.aaccnet.org/meetings/2002/abstracts/a02ma286.asp.

Maranphal, N., M. Barron, M.N. Riaz, L.W. Rooney and R.D. Waniska. 2002. Utilization of Partially Defatted Peanuts in Corn and Flour Tortillas. IFT Annual Meeting and Food Expo, June 15-19, Anaheim, CA.
http://ift.confex.com/ift/2002/techprogram/ paper_13893.htm.

Maranphal, N., M. Barron, L. Rooney, L., R.D. Waniska and M.N. Riaz. 2002. Extrusion properties of sorghum for direct expanded snacks. AACC 87th Annual Meeting, October 13 17, Montreal, Quebec. Quebec.
http://www.aaccnet.org/meetings/2002/abstracts/a02ma347.asp

Silva, L., Rooney, L.W., and Waniska, R.D. 2002. Comparison of two starch stabilization methods in corn tortillas. AACC 87th Annual Meeting, October 13 17, Montreal, Quebec.
http://www.aaccnet.org/meetings/2002/abstracts/a02ma379.asp.

Issues: Sorghum quality for food is alleged to be inferior and sorghum is used only as livestock feed in the Western Hemisphere. Improved sorghum food quality must be developed to capture food and ingredient markets.

Fresh corn tortillas have excellent taste and texture but many consumers have never tasted a fresh wheat tortilla. Methods to maintain texture and taste during storage are needed.

Need to determine the characteristics of flour for tortillas.

What Was Done: New sorghum hybrids with significantly improved food characteristics were developed by incorporation of genetic material from the world collection. We devised methods to evaluate sorghum milling and food properties that were used to select food types of sorghum.

New methods to measure texture and other changes during staling of tortillas were devised. These methods were applied to determine ingredients affecting staling of corn and flour tortillas.

A method to evaluate wheat flour quality for tortillas was standardized.

Impacts: The new sorghums have high yields, and with slightly higher grain prices, producers grow the food sorghums. Value added sorghum products for ethnic and dietary markets are being sold with excellent results. Applications in bakery products, brewing, etc. are feasible with the new grain types. Additional new earlier maturing hybrids are in the pipeline. Information and grain samples were supplied to potential Japanese food processors by US Grains Council. Increased markets for food sorghum are possible.

Usefulness of Findings:

• Snacks from U.S. identity preserved food sorghums are being sold in Japan.

• Food sorghums have excellent extrusion properties and produce bland, light-colored extrudates equivalent to rice. Whole sorghum can be extruded to produce excellent products for special dietary and ethnic needs.

• The understanding of what happens during staling will lead to improved flavor and texture of tortillas which could significantly increase their consumption since they are low in fat and contain significant levels of calcium and fiber.

• Wheat flour quality attributes for tortillas is significantly different from good quality bread flour.


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                                                                                     NC-213 Progress Report for 2002
 
From:     USDA, Agricultural Research Service
              National Center for Agricultural Utilization Research
              Peoria, Illinois
 
By:         Wicklow, D.T.
 
NC-213 Objective:     A                                                                                                                      Procedure:     1a
 
Project Objectives:      Improve corn resistance to Aspergillus flavus and aflatoxin.
 
Results for 2002:          Several corn varieties showing substantial resistance to A. flavus infection or aflatoxin were developed by a University scientist but it was not known if this resistance might be attributed, in part, to the reduction or elimination of seed coat tearing as a factor in corn kernel susceptibility to fungal infection. A University scientist in Champaign, IL, in collaboration with an ARS scientist in Peoria, IL, inoculated corn ears in the milk stage with Aspergillus flavus and then determined the aflatoxin levels for the bright greenish yellow fluorescent (BGYF) infected kernels in the harvested grain. Aflatoxin resistance was attributed to (1) inbred parents that resist seed coat tearing and (2) internal sources of kernel resistance, as demonstrated by infected BGYF kernels showing consistently lower levels of aflatoxin. This information provides for a more accurate interpretation of resistance mechanisms among successful varieties and invites corn breeders to combine these different sources of resistance in producing commercial varieties.
 
The aflatoxin and fumonisin in grain at harvest is found concentrated at high levels in relatively few corn kernels and our research seeks to accurately identify and remove these toxin-contaminated kernels from contaminated grain lots. An ARS Scientist in Manhattan, KS, in collaboration with an ARS scientist in Peoria, IL, obtained near infrared spectra for 2400 kernels from ears inoculated with one of 11 common kernel rotting fungal pathogens, including the mycotoxin producers Aspergillus flavus and Fusarium verticillioides. After spectra acquisition, it was possible to correctly classify kernels infected with these fungal pathogens. The data will be used in building spectral and other features of classification into computer models for automatic detection and segregation using high volume commercial optical sorters for grains and oil seeds.
 
Plans for 2003:        Identify and quantify mold populations and determine aflatoxin and fumonisin levels of grain samples segregated (accepted versus rejected ) using high volume commercial optical sorters. These combine-harvested grain samples come from fields in central Illinois where we wound-inoculated ears with A. flavus or F. verticillioides.  This research involves collaboration with T. Pearson, GMPRC, ARS, Manhattan, KS.
 
Classify grains from wound-inoculated ears of corn varieties with demonstrated resistance or
class
susceptibility to A flavus  kernel rot and/or aflatoxin in 2002 variety trials performed by a corn seed producing company. We seek to determine if the near infrared spectra used in detecting aflatoxin contaminated grains of Pioneer 3394 will prove equally effective in evaluating grains over a diverse genetic background. This collaborative research involves corn pathologists at a seed producing company and T. Pearson, GMPRC, ARS, Manhattan, KS.
 
Describe the antifungal compounds produced by corn endophytes when cultured on autoclaved corn kernels and attempt to detect these metabolites in corn at harvest, in collaboration with J.B. Gloer, University of Iowa.
 
Publications:
Dowell, F.E., T.C. Pearson, E.B. Maghirang, F. Xie, and D.T. Wicklow. 2001. Reflectance and transmittance spectroscopy applied to detecting fumonisin in single corn kernels infected with Fusarium verticillioides. Cereal Chemistry 79: 222-226.
 
Pearson, T.C., D.T. Wicklow, E.B. Maghirang, F. Xie, and F.E. Dowell. 2001. Detecting aflatoxin in single corn kernels by using transmittance and reflectance spectroscopy. Transactions of the ASAE 44: 1247-1254.
 
Issues: In the Midwestern corn belt, the bulk of the U.S. corn crop is at risk during sporadic outbreaks of aflatoxin contamination of preharvest corn (Zea mays L.). Aflatoxin is a metabolite produced by the fungus Aspergillus flavus. The overall goal of this research program is to attempt to control A. flavus infection of pre-harvest corn through an integrated approach to disease management.  Procedure 1a evaluates corn genotypes for resistance to kernel infection and aflatoxin.
 
What Was Done: Corn varieties with demonstrated aflatoxin resistance or susceptibility were examined for numbers of bright greenish yellow fluorescent (BGYF) kernels and the aflatoxin content of those kernels. This approach allows corn pathologists to distinguish sources of resistance linked to seed coat integrity or the seed proper (germ and endosperm) and invites corn breeders to combine these different sources of resistance in producing commercial varieties.
 
Impacts: The ability to accurately classify individual aflatoxin or fumonisin contaminated kernels, including those with symptomless infections presents opportunities for detection of these grains in a variety of applications including the rapid evaluation of grain samples from corn variety trials for mycotoxin resistance.
 
 
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                                                                                     NC-213 Progress Report for 2002
 
From:     Purdue University
              Agricultural & Biological Engineering
 
               Kansas State University
               Grain Science & Industry
 
By:          Maier, D.E.
               Herrman, T.
               Berruto, R.
               McGill, J.M.
 
NC-213 Objective:      A                                                                                                                             Procedure:     2
 
Project Objectives:       The main goal of this project is to use the system simulation approach to improve the efficiency and economics of the receiving operation at commercial grain elevators.
 
Title:     System modeling of grain handling operations.
 
Results for 2002:         The Illinois Grain and Feed Association Annual 2000-2001 Directory was used as the source from which a representative sample of commercial grain elevators was drawn based on warehouse-licensed storage capacity. Six elevators had less than 500,000 bu storage capacity, six elevators had between 500,000 bu and 1 million bu capacity, 15 had between 1-2 million bu capacity, and six had more than 2 million bu capacity. In order to determine the potential for grain segregation, the capacity of conveying equipment in the receiving system was examined at each elevator. The majority of elevators (78%) had at least two receiving pits, which greatly enhances the potential for successful grain segregation. Bucket elevator capacities ranged from less than 5,000 bu/h up to 20,000 bu/h. Thus, Illinois grain handling facilities have the capability to segregate multiple incoming grain streams. In terms of storage structures, 65% of surveyed elevators had more than ten bins with less than 50,000 bu capacity. The ratio of bins less than 50,000 bu to those greater was nearly 4:1. Thus, storage of segregated grain should not be a problem either. The inability to successfully segregate multiple streams of incoming grains could generally be related to poor management of incoming trucks during the harvest peak period, lack of qualified personnel and poor allocation of resources.
 
Plans for 2002:     Develop additional facility-specific models and use them in order to study unloading strategies for commodity grains at commercial grain handling facilities.
 
Perform simulation runs to evaluate the impact of IP and GM grains and oilseeds on unloading operations, and simulate logistics changes to improve system performance.
 
Issues: Increased globalization of agricultural markets and the introduction of transgenic (GM) crops through biotechnology have created a need for further investigation of the U.S. grain handling infrastructure to segregate cereals and oilseeds in order to remain the world’s least cost provider of safe and wholesome foods and feeds. This research addresses the study of grain unloading operations at country elevators using system simulation methodology. The differentiation of crops (Transgenic crops, Identity Preserved crops, etc.) increases the burden of elevators, which need to handle the same or greater amounts of grain, but with an increased number of crop types to be segregated. This fact poses a significant challenge for operators in terms of unloading, drying, and storage of different grains in facilities that were built to handle few commodities (corn, wheat and soybeans).
 
What Was Done: The goal of this project is a system simulation study of country elevators to improve the efficiency and the economics of grain handling. The study, rather than addressing the economic value of the operation with respect to IP or non-GM grains, will analyze different simulated scenarios (product arrival, logistic of the system, management strategies, external drivers of change like market forces and regulations) in order to provide strategies to better manage the grain receiving operation.
 
Impacts: Creation of an object oriented grain handling system software package will enable elevator operators to test the flexibility of their current facility or create their ideal grain handling facility with all its equipment in a simple click and drag type format. A system simulation and economic analysis tool would allow them to define the operating conditions at their elevator (e.g. truck size distribution, type of grain, delivery rate), estimate average delay time and queue length, and quantify the least cost segregation strategy. Additionally, this approach holds the future potential for a grain company (or merging companies) to link multiple individual elevator facility models together and evaluate the optimization of combined system network resources.
 
Funding Sources:
Anderson Team Research Grant Program 2000-2002
 
Contacts:
Dirk Maier, Department of Agricultural and Biological Engineering, Purdue University, Phone: 765-494-1175; Fax: 765-496-1356; e-mail: maier@purdue.edu
 
Tim Herrman, Department of Grain Science & Industry, Kansas State University, Phone: 785-532-4082; Fax: 785-532-4017; e-mail: tjh@wheat.ksu.edu
 
URL:     http://www.GrainQuality.org
 
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                                                                                     NC-213 Progress Report for 2002
 
From:     Kansas State University
              Department of Grain Science and Industry
 
By:         Herrman, T.J.
              Loughin, T.
              Knapp, M.
 
NC-213 Objective:   A                                                                                                              Procedure:   1b, 2a
 
Title:     Pre-harvest Prediction of Kansas Wheat Protein Content
 
Project Objectives:     Explore the feasibility for predicting wheat protein content prior to harvest.
 
Results for 2002:        Advance knowledge of the protein content of the Kansas wheat crop can facilitate purchasing, marketing, and logistic decisions within the state’s grain industry. The first of two studies involved development of pre-harvest protein and dough factor prediction models utilizing Kansas State University’s wheat performance plots at 6 locations and included 8 cultivars. Models for Zadok’s growth stage 40+5 and 60+5 were developed using two years’ data and then tested using the third year’s data. The adjusted coefficients of determination (R2) for the two models were 0.84 and 0.81, respectively, using variety, weather, and leaf chlorophyll content as model regressors. In the second study, two pre-harvest protein prediction models (pre-heading and post-heading) were created using Kansas Agricultural Statistics Service’s (KASS) objective yield survey samples from approximately 250 commercial fields per year for 6 years (1997-2002). The prediction models were created using plant, location, variety, and precipitation data from county level National Weather Service stations. These models were validated by withholding 40% of the data while modeling and using these data to test the models. The models developed with the training data set displayed average predictive capability for central and western Kansas crop reporting districts before (R2 = 0.78) and after (R2 = 0.82) heading. The validation data set used to predict district level average crop protein resulted in R2 values of 0.30 before and 0.48 after heading. The state level prediction before and after heading resulted in R2 values of 0.81 and 0.91 for the validation data set. The standard error for the first (0.17) and second (0.19) state predictions, calculated at a 95% confidence level, indicates good model precision.
 
Plans for 2003:
Seek commercial support for this program. Repeat the prediction for 2003 Kansas wheat protein content.
 
Publications:
Herrman, T.J., T.M. Loughin, M. Knapp, and R. Kaldate. 2002. Pre-harvest prediction of Kansas wheat protein content. Crop Science. Submitted.
 
Issues:        State level pre-harvest protein predictions and, to a lesser extent, district level pre-harvest protein predictions, display sufficient accuracy to help grain industry personnel manage year to year protein variability in the Kansas wheat crop.
 
Impacts:     The value of protein is determined, in part, by the supply of protein. The prediction of the protein potential in the Kansas wheat crop prior to harvest can facilitate purchasing, marketing, and logistic decisions within the grain industry.
 
Funding Sources:
The Grain Industry Alliance
 
Contacts:
Tim Herrman, Department of Grain Science and Industry, phone: (785) 532-4082, tjh@wheat.ksu.edu
 
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                                                                                     NC-213 Progress Report for 2002
 
From:     Kansas State University
 
By:         Herrman, Tim
              Shroyer, Jim
              Lee, Kyung Min
              Lingenfelser, Jane
 
NC-213 Objective:    A                                                                                                                                             Procedure:   2a
 
Title:     Improve hard white (HDWH) wheat yield and quality through identifying the blend of two varieties (Trego and Betty) that optimizes agronomic and processing performances.
 
Results For 2002:    We analyzed samples from three of the four locations where we planted a Betty:Trego blend. The three locations included Tribune, Colby, and Hays. We measured single kernel properties, protein content, milling performance, and loaf volume of baked bread. The loaf volume results are presented as a percentage of Trego using the following formula:
 
Mix of Betty:Trego – Trego   x 100
Trego
 
Figure 1. The difference in loaf volume between Betty and Trego blends across all three locations, expressed as a percent of Trego.

 
 
 
We observed a significant interaction (P=0.002) between location and mixture treatments for grain yield. Figure 2 presents the yield difference in bushels between Trego and Betty using the formula:
 
Mix of Betty:Trego – Trego
 
The results for 2001 indicate that grain yield was higher with Betty:Trego blend compared to 100% Trego at Hays and Tribune. The grain yield response appears to coincide with the presence of stripe rust, although disease readings were not performed and no statistical analysis was conducted to confirm this relationship in the mixture test plots.
 
For the grain protein response, a location by Trego:Betty blend did not appear significant, so results are expressed across the three locations. The blending of Trego:Betty was significant (P<.0001).
 
Figure 2. The difference in grain yield (bu) between Betty:Trego blends and Trego for Colby, Hays, and Tribune.
 
 


 
 
 

Figure 3. The difference between percent protein between Betty:Trego blend and Trego.



Results for 2001 indicate that blending wheat cultivars of similar properties and different yield potential and end-use properties can display an additive effect and enhance value for Kansas farmers.


 
2002 Yield and Protein Results
 
The grain yield and percent protein responses to Betty:Trego blends are presented in Figures 4 and 5. A significant (P<.0001) grain yield response to Betty:Trego blend and a significant (P=.004) protein response to the location by blend interaction were observed.
 
Figure 4. The difference in grain yield between Betty:Trego blends and Trego.





Figure 5. The difference in percent protein between Betty:Trego blends and Trego for Colby, Hays, Tribune, and Garden City.

 
Summary:     Blending hard white wheat to maximize grain yield and protein content appeared to be a sound strategy in 2001, while planting 100% Trego appeared to be significantly more profitable based on 2002 grain yield and protein results. We are repeating the experiment a third year, due to the very different growing seasons and results observed during 2001 and 2002.
 
Impacts:       HDWH wheat commercialization offers Kansas wheat producers an opportunity to capture added value resulting from its preferred seed coat color. The current world market suggests that HDWH wheat can trade for $10 per mt higher than red wheat. Based on the current genetic capabilities of HDWH wheat, a potential for 3 million acres (about 2.5-3 million mt) of production exists in western Kansas.
 
Issues: Wheat acres in Kansas declined from 10.1 million in 1998 to approximately 8.1 million harvested acres in 2002. The value of the Kansas wheat crop ranged between $1.26 billion and $900 million annually during the period. The western third of Kansas is well suited to the adoption of HDWH wheat. The annual flour production in Kansas is approximately 42 million cwt worth $383 million, which is first in the nation.
 
Barriers to the successful commercializing of HDWH wheat were identified during focus group sessions conducted in western Kansas by Grain Science Extension and Agronomy Extension specialists. Farmers identified the current economic climate as the main reason that made them averse to any form of risk including planting wheat with a different color seed coat. Other limitations included limited choice of proven cultivars and seed availability. The HDWH wheat variety Betty tends to display higher protein content compared to Trego while Trego displays superior yield potential. Combining these traits into a single field through blending seed to pre-determined ratios offers the possibility of achieving high yield, good protein content, and good protein quality.
 
Thus, the purpose of this study involves identifying seed blends of Trego and Betty HDWH wheat cultivars that optimize yield and quality in an attempt to maximize profitability for Kansas wheat farmers, speed adoption of HDWH, and minimize producer risk resulting from limited commercial varieties adapted for western Kansas.
 
Funding Sources:
Kansas Wheat Commission and Kansas Dept. of Commerce and Housing-Ag. Product Development Division, KAES
 
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                                                                                     NC-213 Progress Report for 2002
 
From:     USDA, Agricultural Research Service
              National Center for Agricultural Utilization Research
              Peoria, Illinois
 
By:         Wicklow, D.T.
              McAlpin, C.E.
 
NC-213 Objective:    A                                                                                                                             Procedure:     2a
 
Project Objectives:     Investigate the microbial ecology and epidemiology of Aspergillus flavus in Midwestern crop field soils and the biocontrol potential of sclerotial mycoparasites.
 
Results for 2002:         Evidence is presented which demonstrates a relationship between strength of the vegetative compatibility reaction and aflatoxin production in wild-type isolates of A. flavus and A. parasiticus using the suspended disc culture method (R.A. Norton, 1995). Combining aflatoxin-producing isolates belonging to different vegetative compatibility groups (VCGs) resulted in a substantial reduction in aflatoxin yield. Pairs of aflatoxin-producing isolates within the same VCG, but showing weak compatibility reactions using complementary nitrate-nonutilizing mutants, also were associated with reduced levels of aflatoxin B1. In contrast, pairings of isolates displaying a strong compatibility reaction typically produced high levels of aflatoxins. These results suggest that interactions between vegetatively compatible wild-type isolates of A. flavus or A. parasiticus are cooperative and result in more aflatoxin B1 than pairings between isolates that are incompatible.
 
Aspergillus bombycis is a recently described aflatoxin producing species known only from domesticated silkworm culture in Southeast Asia and Japan, and there is a need to determine if one or more ‘domesticated’ forms of A. bombycis are common to such facilities having been spread with the transfer of diseased silkworms. A scientist from the Ministry of Agriculture, Forestry and Fisheries in Tsukuba, Japan, in collaboration with an ARS scientist in Peoria, IL, performed isolations of A. bombycis from silkworm rearing facilities, determined the ability of each isolate to produce aflatoxins and related mycotoxins, and then subjected each isolate to DNA fingerprinting. Among 18 isolates analyzed, each of which produced B and G aflatoxins, seven distinct DNA fingerprint groups were identified, including GTAb-2 isolated from silkworm rearing facilities in four Prefectures of Japan. Aflatoxin has been linked to increased virulence in Aspergillus disease of silkworms and the results of this study suggest a selection for aflatoxin producing ability among domesticated A. bombycis populations which may parallel the origin of sericulture in China about 2700 B.C.
 
There is an urgent need for new sources of antifungal agents and fungi that parasitize and kill other fungi offer a potential source of novel antifungal agents useful to agriculture and medicine. Using the two most important mycotoxin producers A. flavus and F. verticillioides as targets, a University scientist in Iowa City, IA, in collaboration with an ARS scientist in Peoria, IL, have isolated and identified numerous antifungal metabolites present in culture extracts of fungi that grow on larger fungi. These ongoing studies are contributing to a growing data base that will be useful in interpreting structure function relationships and potential cellular targets in Aspergillus and Fusarium. All novel bioactive compounds discovered in this research are being further tested by the animal health and pharmaceuticals industry.
 
Plans for 2003:     Examine the potential role of intraspecific competition in naturally suppressing aflatoxin outbreaks in the central U.S. Corn Belt. Evaluate the abilities of non-aflatoxin producing biocontrol strains of Aspergillus flavus and Aspergillus oryzae  to interfere with aflatoxin production in preharvest corn kernels. Isolate and identify mycoparasitic fungi, produce fermentation extracts, and perform bioassays using Aspergillus flavus and Fusarium verticillioides to guide the isolation of novel antifungal metabolites.
 
Publications:
Che, Y., J.B. Gloer, and D.T. Wicklow. 2002. Phomadecalins A-D and phomapentenone A: New metabolites from Phoma sp. NRRL 25697, a fungal colonist of Hypoxylon stromata. Journal of Natural Products 65: 399-402.
 
Holler, U., J.B. Gloer, and D.T. Wicklow. 2002. Biologically active polyketide metabolites from an undetermined fungicolous hypomycete resembling Cladosporium. Journal of Natural Products. 65: 876-882.
 
Li, C., J.B. Gloer, D.T. Wicklow and P.F. Dowd. 2002. Thiersinines A and B: Novel antiinsectan indole diterpenoids from a new fungicolous Penicillium species (NRRL 28147). Organic Letters 4: 3095-3098.
 
McAlpin, C.E., D.T. Wicklow and B.W. Horn. 2001. DNA fingerprinting analysis of vegetative compatibility groups in Aspergillus flavus from a peanut field in Georgia. Plant Disease 86: 254-258.
 
Wicklow, D.T., C.E. McAlpin and S.W. Peterson. 2002. Common genotypes (RFLP) within a diverse collection of yellow-green aspergilli used to produce traditional Oriental fermented foods. Mycoscience 43: 289-297.
 
Issues:     In the Midwestern Corn Belt, the bulk of the U.S. corn crop is at risk during sporadic outbreaks of aflatoxin contamination of preharvest corn (Zea mays L.). Aflatoxin is a metabolite produced by the fungus A. flavus. The overall goal of this research program is to attempt to control A. flavus infection of pre-harvest corn through an integrated approach to disease management. In Procedure 2a we examine the origins of fungal infective inoculum in corn fields and management practices to prevent a population build-up leading to mycotoxin contamination. A promising strategy for eliminating preharvest aflatoxin of susceptible crops, especially in environments conducive to an aflatoxin outbreak, involves field applications of non-aflatoxin producing strains of A. flavus or A. parasiticus, which displace the naturally occurring aflatoxin-producing strains
class
through mechanisms of intraspecific competition. There is a need to explain the underlying mechanism(s)of competition and this study describes a novel procedure for evaluating Aspergillus interference with aflatoxin production.
 
What Was Done: Aflatoxin producing isolates of Aspergillus flavus showing different abilities to fuse with one another were paired in laboratory culture and examined for total fungal growth (mycelium dry weight) aflatoxin content. The results suggest that interactions between vegetatively compatible wild-type isolates of A. flavus or A. parasiticus are cooperative and result in more aflatoxin B1 than pairings between isolates that are incompatible.
 
Impacts: Intraspecific competition is the basis for biological control of aflatoxins and there is a common interest in understanding the mechanism(s) by which competing strains of Aspergillus species inhibit aflatoxin production. This information will stimulate new research on how incompatibility reactions function in suppressing aflatoxin production.   
 
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