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| Author(s): | Ehlers R.-U., Lunau S., Krasomil-Osterfeld K., Osterfeld K.H. |
| Year: | 1998 |
| Title: | Liquid culture of the entomopathogenic nematode-bacterium complex Heterorhabditis megidis/Photorhabdus luminescens |
| Citation: | BioControl. 43:(1) 77-86. . |
Abstract:
A process technology for production of the biocontrol agent complex Heterorhabditis megidis/Photorhabdus luminescens in monoxenic liquid culture in laboratory scale bioreactors is described. Dauer juvenile yields varied between 21 and 68 million dauer juveniles/litre medium. The maximum density was reached at 13 to 25 days after inoculation of P. luminescens. The reason for the high variability in yield was identified. After the 24 h bacterial preculture the bioreactor was inoculated with nematode dauer juveniles which develop to self-fertilizing hermaphrodites. The exit from enduring dauer stages (recovery) was between 18 and 90% of the inoculum density. Low dauer juvenile recovery resulted in the development of two-generations within 20 to 25 days. In contrast, high dauer juvenile recovery led to a one-generation process terminated within 15 days. Factors influencing dauer recovery are still unknown |
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