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| Author(s): | Jagdale G.B., Gordon R. |
| Year: | 1998 |
| Title: | Variable expression of isozymes in entomopathogenic nematodes following laboratory recycling |
| Citation: | Fundamental and Applied Nematology. 21:(2) 147-155. . |
Abstract:
Cellulose acetate electrophoresis was used to determine the degree to which isozyme banding patterns differed among four strains of steinernematid nematodes that had been recycled or stored at 5, 10, 15, 20 and 25 degrees C: Steinernema feltiae Umea strain, S. carpocapsae All strain, S. riobravis TX strain and S. feltiae NF strain. In all four strains, isozyme banding patterns of malate dehydrogenase (MDH), mannose- 6-phosphate isomerase (MPI) and phosphoglucomutase (PGM) were affected by recycling or storage temperatures. The NF and Umea strains of S. feltiae synthesized additional isozymes of MPI and MDH or PGM at cold temperatures, while S. carpocapsae All strain synthesized three isozymes of MDH at warm tempe ratures and an additional isozyme of PGM at cold temperatures. The implications of these findings are discussed in relation to temperature adaptation mechanisms involving synthesis of isozymes and the use of isozyme determinations in steinernematid taxonomy |
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