GFP in Developing Embryos

 

Transformed D-20 tissue

 #2t.bmp (1340982 bytes)#2t GFP.BMP (1340982 bytes)
white light                    blue light

 

Transformed Developing embryos

 White Light            Blue Light

#2-4.bmp (1340982 bytes)

#2-4 GFP.BMP (1340982 bytes) 

Low Magnification

#2-6.bmp (1340982 bytes)

#2-6 GFP.BMP (1340982 bytes)

High Magnification

       At low magnifications GFP is difficult to detect, but as the magnification is increased, GFP becomes visible.

 

#2-7.bmp (1340982 bytes)#2-7 GFP.BMP (1340982 bytes)

These two images are from the same clump of transformed tissue as above, but in this area, the transformed, bright green tissue appears red under blue light due to chlorophyll autofluorescence.
 

Developing embryos on M-6 - no charcoal
Non-transformed
#3.bmp (1340982 bytes)#3 GFP.BMP (1340982 bytes)  #3-2.bmp (1340982 bytes)#3-2 GFP.BMP (1340982 bytes)

#6.bmp (1340982 bytes)#6 GFP.BMP (1340982 bytes)  #6-2.bmp (1340982 bytes)#6-2 GFP.BMP (1340982 bytes)

The dried edge of the cotyledon in the bottom row might be mistaken for GFP under blue light, but when compared side by side with GFP expressing tissue under the microscope, a difference can be distinguished.

Transformed
#4-2.bmp (1340982 bytes)#4-2 GFP.BMP (1340982 bytes)  #4-4.bmp (1340982 bytes)#4-4 GFP.BMP (1340982 bytes)

#4-6.bmp (1340982 bytes)#4-6 GFP.BMP (1340982 bytes)  #4-5.bmp (1340982 bytes)#4-5 GFP.BMP (1340982 bytes)

The two rows above are images of the same embryo while the row below is a different embryo.

#2tt.bmp (1340982 bytes)#2tt GFP.BMP (1340982 bytes)  #2tt-2.bmp (1340982 bytes)#2tt-2 GFP.BMP (1340982 bytes)

In the last row of images, GFP is masked by chlorophyll autofluorescence.   However, when viewed through the microscope, several spots of GFP can be seen at the highest magnification.  For example, in the image on the right, a few faint spots of GFP are present, but the camera does not pick up the GFP fluorescence.