MS Salt Stock (per liter)
B-5 Vitamin Stock (per liter)
MS Sulfate 100x stock (g/l)
MgSO4·7H2O 37.0
MnSO4·H2O 1.69
ZnSO4·7H2O 0.86
CuSO4·5H2O 0.0025
MS Halides l00x stock (g/l)
CaCl2·2H2O 44.0
KI
0.083
CoCl2·6H2O 0.0025
MS P,B,Mo l00x stock (g/l)
KH2PO4
17.0
H3BO3
0.62
Na2MoO4·2H2O 0.025
MS FeEDTA l00x stock (g/l)
Na2EDTA
3.724 first
FeSO4·7H2O 2.784
dissolve overnight in dark bottle with stirring
B-5 vitamins 100x stock
10 g m-inositol
100 mg Nicotinic Acid
100 mg Pyridoxine·HCl
1 g Thiamine
store in dark bottle
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497; 1962.
Gamborg, O.L.; Miller, R.A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exper. Cell Res. 50:150-158; 1968.