Soybean Defense Gene Silencing Project

A Collaborative Effort of the Graham Lab (Ohio State) and Yu Lab (Danforth Center)

Our collaboration takes advantage of the extensive experience of the Graham lab in studying soybean defense responses and recent developments in soybean gene silencing protocols in the Yu lab.  Soybean cotyledons are a very attractive tissue in which to study cellular aspects of defense responses to elicitor and infection.  A critical early finding from our collaborative work is that Agrobacterium-mediated gene silencing of transformed roots [initiated from cotyledons (A&B below)] moves into the cotyledon, allowing us to use this highly characterized cotyledon system to knock out key genes (GUS knockout shown, C) and test the many hypotheses on the key signaling and defense genes in this system.  The links below describe some details of our collaborative work. 

                                                                                             A                                                            B                                                                           C            

 Working models                           Defense genes of interest                            Gene silencing protocols

Other Links: Graham lab / Graham pubs / Yu lab / Yu pubs