Protocols
Acrylamide Gels
Agarose Gels
Alfalfa Vegetative Propagation and Growth
Arabidopsis Growth and Genetic Transformation
b-Galactosidase Assays
Bradford Assays
CaCl2 Transformation of E. coli
Competent Cell Preparation (CaCl2)
Competent Cell Preparation (Electrocompetent)
Dialysis Tubing Preparation
DNA Isolation (Plant Genomic DNA)
Electro Transformation of E. coli
Formamide Deionization
Fusion Protein Production (GST & HIS Tagged Proteins)
Genomic (Plant) DNA Restriction Digestion & Southern Hybridization
Bacteriophage l Genomic DNA Library (construction through subcloning inserts)
Glycerol Stock Preparation and Maintenance
GUS Assays
GUS Histochemical Staining
Helper Phage Production
In Vitro Site Directed Mutagenesis
In Vivo Excision of Plasmid DNA from Bacteriophage λ
Laemmli Gels
Medicago truncatula Seed Extraction, Germination and Vernalization
Nucleotide Preparation
Oligonucleotide Labeling
Phenol Extraction and Ethanol Precipitation of DNA
Phenol Preparation (Buffering)
Pipette (Glass) Washing Procedure
Plasmid DNA Preparation (CsCl2)
Plasmid DNA MiniPreps
Protein Electroblotting
Protein Isolation
Restriction Digests
Rice Growth
RNA Isolation for cDNA Library Construction
RNA Gels
RNA Hybridization
Sephadex Column Preparation
High Throughput DNA Sequencing: Bacterial Culture Preparation
Shotgun Library Construction (A multi-step procedure that includes vector preparation)
Taq Polymerase Synthesis
Tomato Seed Harvesting and Cleaning
Tricine Gels
Yeast One Hybrid Screening (See Complete Course Manual)
Yeast Transformation
