Laboratory Exercise Schedule (2005)

Tue. March 29             Introduction, Lab Safety,

and note book

Thur. March 31            Recombinant DNA and DNA Ligation

Tue. April 5                  Bacterial transformation

                                                April 6, start overnight cultures

Thur. April 7                 Plasmid DNA minipreps

Tue.  April 12               Restriction digest analysis

and preparation of samples for sequencing

Thur.  April 14              Genetic Resources on the Web

                                    Notebook grading

Tue. April 19            Construction of DNA libraries

                                        General Background

                                         Specific Background

                                         Identification of positive BAC pools protocol         

Thur. April 21           Identification of positive BAC pools 

                                          Titration            

Tue. April 26            Preparation of high density BAC filters 

Thur.April 28            Identification of positive clones

                                    Notebook grading

Tue. May 3               Genetic analysis lesson I (DNA extraction -CTAB)

                                                Alternative protocol 96-well plate extraction

Thur. May 5              Genetic analysis lesson II (sequencing)

Tue. May 10             Genetic analysis III (analysis)

Thur. May 12            Genetic analysis IV (analysis)

Notebook grading

Tue. May 17            Gene expression analysis – RNA extraction

Thur. May 19           Gene expression analysis by RT-PCR

Tue May 24             Gene expression analysis by microarray

                                    Notebook grading

Thur.May 26                Protein Expression in E-Coli 

Tue. May 31                Protein Purification

Thur. June 2                 Enzyme activity optimization 

                                    Notebook grading