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Day 1

Survey (WORD DOC)

Introduction (ppt)

Downloading Custom Data sets (ppt)

Data:      TA496 ESTs FASTA (warning: won't download)

                TA496 ESTs text (warning: 76 Mb)

                phtyphthora - tomato interaction FASTA

                phytphthora - tomato interaction text

                random tomato sequence text file

Day 2

Installing a UNIX emulator on your WINDOWS machine (ppt)

 

UNIX in MAC OS X

 

Day 3 -   NOTE WE WILL MOVE THE CLASS TO GOURLEY HALL

                SECOND FLOOR CONFERENCE ROOM (206).

 

Running Linux in Windows (ppt)

 

Linux Cheat Sheet

 

Day 4

Installing BLAST (ppt)

Day 5

Week 2 Introduction

(Re-installation of Cygwin with PERL [use full installation])

Day 6
Introduction to PERL and BioPERL (Includes installation of BioPERL)

Test Script

Day 7

BioPERL Modules

Sequence file
BioPERL Script to format sequence

Day 8

Parsing BLAST outputs (ppt)

PERL script for parsing BLAST outputs
Sample BLAST output

Day 9

BLAST options

Protein data
    SB6-94-4
    SB7-15-13
    SB7-15-13Pep4A

Parsing BLAST outputs - Script modification
   New Parsing PERL script

Day 10

    BLAST Practice
    PERL script for retrieving accessions identified in AraCyc database

Day 11

    Parsing BLAST (see day 8, practice, practice, practice)

   Using the R statistical Package

   PERL script for GC content

Day 12

    Finish GC content excercise
    Post-Class Survey
    Discussion

References:

Mount, David W.  2004.  Bioinformatics: Sequence and Genome Analysis, second edition. Cold Spring Harbor Laboratory Press, NY.

Danesh, A and Jan, M. 2001. Mastering Linux. Red Hat Linux 7. Publisher's Edition. 2nd ed.
 
Barkakati, N and Wall, K. 2002. Red Hat Linux 7.2. Weekend crash course. Hungry minds. P. 72-73, 85-86.

 

Holzner, S. 2001. Perl Black Book. Coriolis. 2nd edition.

 

Schwartz, R. and Phoenix, T. 2001. Learning Perl. O'Reilly & Associates, Inc. Third edition.
 

Tisdall, J. 2003. Mastering Perl for Bioinformatics. O'Reilly & Associates, Inc.First ed.
 

Wall, L., Christiansen, T. and Orwant, J.  2000. Programming Perl, 3rd Edition.  O'Reilly Media, Inc

OhioLink allows OSU computers to access O'Reilly texts online:    PERL on line